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COOMBS TESTİ - İNDİREKT


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Medicine and Hematology Glossary

COOMBS TESTİ - İNDİREKT

(Medicine and Hematology Glossary) :
Serumda dolaşan serbest antikorları saptamak üzere yapılır. Hastadan kan alınarak serum ayrılır. Bu serum başka bir kaynaktan elde edilen O Rh(-) eritrositlerle inkübe edilir. Daha sonra bu eritrositler coombs serumu ile karşılaştırılır. Eğer hasta serumunda serbest antikor varsa inkübe edilen eritrositlere bağlanacak bunlar da coombs serumundaki antikorlarla karşılaştırıldıklarında agglütinasyon göstereceklerdir. İndirek coombs testinin esas kullanım alanı kan bankacılığıdır. Rh uyumsuz bebekleri olan annelerin serumlarındaki antikorlar da indirekt coombs testi ile saptanır.
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